Microscope Illumination System | CoolLED

Comparing microscope illumination systems. Whether using fluorescence microscopy or transmitted light methods, the light hitting a sample can make the ... Skiptocontent UK+44(0)1264323040USA508.599.0447 Search: Search FacebookpageopensinnewwindowTwitterpageopensinnewwindowLinkedinpageopensinnewwindowYouTubepageopensinnewwindowWechatpageopensinnewwindow HomeAboutUsAboutUsMeetTheTeamCoolLEDMissionProductsProductRangeProductComparisonpE-800SeriespE-800pE-800furapE-4000pE-300SeriespE-300litepE-300whitepE-300ultrapE-340furapE-100pT-100OEMIlluminationAccessoriesAdaptorsSupportSupportWhyLEDIllumination?VideoResourcesOpticalFiltersImagingSoftwareEnvironmentHealthandSafetyWarrantyTechnicalResourcesNews&EventsNewsEventsNewslettersContactContactUsProductRegistrationLocalResellersResellerLogin Comparingmicroscopeilluminationsystems Whetherusingfluorescencemicroscopyortransmittedlightmethods,thelighthittingasamplecanmakethedifferencebetweenabreakthroughdiscoveryandinconclusiveresults.Selectingtheoptimalmicroscopeilluminationsystemisthereforecrucial.Untilrecently,choiceswerelimitedtomercuryormetalhalideforwidefieldfluorescenceorhalogenlampsfortransmittedlight.SinceLEDmicroscopeilluminationsystemsarrivedonthemarket,scientistsaroundtheglobeareincreasinglymovingtowardsthistechnologyduetoitsmanybenefits.WelookatwherethetechnologyistodayandcompareLEDmicroscopeilluminationsystems,tohelpmicroscopistsselecttheoptimumsystemfortheirneeds. CoolLEDandmicroscopeilluminationsystems CoolLEDdesignsandmanufacturescuttingedgeLEDmicroscopeIlluminationSystemsforresearchersandcliniciansusingthelatestLEDtechnology. SinceourteamoffourintroducedthefirstcommerciallyavailableLEDmicroscopeilluminationsystemforfluorescencemicroscopyin2006,wehaveledthewayinilluminationtechnologyforfluorescenceandtransmittedapplications.Nowweareafast-growingcompanyinHampshire,UK,withavastproductrangeandtechnicalexpertisespanningopticalengineeringandthelifesciences. Wealsounderstandthatsomethingoutoftheordinaryissometimesrequired,andmanufacturerswishingtogainacompetitiveadvantagecannowaccessourworld-renownedLEDtechnologyincustomisedconfigurationsat www.OEMIllumination.com. UK:+44(0)1264323040 USA:1.800.877.0128 CallUsNow–We’reHereToHelp Whencallingitwouldbeusefultohavethefollowinginformation: YourName CompanyName(IfApplicable) EmailAddress YourMicroscopeIlluminationSystemRequirements RequestACallback Amemberofourteamwillcontactyouwithinthenexttwoworkingdays LEDversusmercuryandmetalhalidemicroscopeilluminationsystems Forwidefieldfluorescencemicroscope,LEDmicroscopeilluminationsystemsoffermanybenefitscomparedtoconventionalmercuryandmetalhalidelamps: Performance:LEDsaremuchmorestableovertime,whereasirradiancedeclinesoverthelifetimeofamercuryormetalhalidelamp.Historically,theirradianceofLEDswasconsideredlow,andalthoughthiscanstillhauntitsreputation,thetechnologyhasnowmaturedandLEDsareequivalentorbrighterinmanyareasofthespectrum.AsthegrowingefficiencyofLEDsleadstoirradiancegains,LEDsnowtypicallyexhibitabove30%efficiency,whereastraditionallampsaremostlybelow10%.Asefficiencyincreasesthisalsoreducesheatoutput,whichhasanaddedbenefit,sincereducingoperatingtemperatureisknowntoincreaseLEDlifetime.BoostingirradianceandextendingthelifetimethroughthermalmanagementissuchasignificantfactorwhendesigningLEDIlluminationSystems,CoolLED’sworkfocusesonarangeofinnovationsinthisarea–forexample,reducingLEDtemperaturesthroughuniqueheatsinkdesignswhichconductheatawayfromtheLEDmuchmoreefficiently. Cost:AlthoughtheupfrontcostishigherforswitchingtoLEDmicroscopeilluminationsystems,thelong-termcostislowerandasavingof£30,000couldbemade(readourthirdpartyreport here).ThisisduetothelongerlifetimeofLEDsandlackofconsumablereplacementsneededasinthecaseoflamps.Specialistdisposalchargesduetothehigh-pressurelampandmercurycontentarealsoavoided. Control:Beingsolid-state,LEDscanbeeasilycontrolled.Theycanbeswitchedonandoffwithprecisemicrosecondtiming,whichremovestheneedforamechanicalshutterandimprovesthetemporalresolutionofexperiments.WithTTLtriggeringandinlinefilters,speedsofupto10µscanbeachieved.LEDirradiancecanalsobemodulatedelectronically,balancingbrightnesswhileminimisingphototoxicityandphotobleaching,withouttheneedforneutraldensityfilters. Convenience:Lampsfrequentlyneedreplacing,whereasLEDsarelong-lived.Thealignmentrequiredafterreplacinglampsistricky,butLEDmicroscopeilluminationsystemscanbefactoryalignedandarereadytobefittedtothemicroscope.Itisbecomingincreasinglydifficulttodisposeofmercuryandmetalhalidelamps.Infact,somecountriesarebanningmercury. Safetyandtheenvironment:Mercuryinlampsishazardoustopeopleandtheenvironment.Lampsarealsoleftonfordaysduringexperiments,whereasLEDscanbeswitchedoffeasilywhennotinuse,whichreducesenergyconsumption.Inadditiontothis,LEDsaremoreenergyefficientandemitmuchlessheat.AdoptingLEDmicroscopeilluminationsystemscanhelplaboratoriesbecomemoresustainable,savingenergyandreducingthecarbonfootprintwhencomparedwithusingmercuryormetalhalidelamps. Sincetheenergycostisjustafractionofthatusedbymercuryandmetalhalide,manyinstitutionsoffer“GreenGrants”tosupportthepurchaseofenergy-efficientequipment.Ifyouwanttofindoutmoreaboutthesustainablebenefits,watchthisinterviewfromAnnaLewisattheUniversityofBristol: CoolLED’sMicroscopeIlluminationSystemProductRange LEDlightingsystemsareavailableforfluorescencemicroscopyandtransmittedlightapplications. Takealookatourproductrangetodiscoverwhichsystemisbestsuitedtoyourrequirements. pE-800fura pE-800 pE-4000 pE-300white pE-300lite pE-340fura pE-300ultra pE-100 pT-100 OEMIllumination Accessories Adaptors Comparingmicroscopeilluminationsystemsfortransmittedillumination Asisthecasewithfluorescencemicroscopy,LEDmicroscopeilluminationsystemsalsoofferimprovedcontrolwhencomparedtotraditionalhalogenlampsfortransmittedlightapplications,withinstanton/offandirradiancecontrolin1%steps. LaboratoriesfamiliarwithhalogenlampswillfindthatthebroadwhiteofmodernLEDmicroscopeilluminationsystems,suchastheCoolLEDpT-100WHTachievesresultswhichmakethemovetoLEDssimpleandapparent,andthisreproducibilityisidealforpathologyapplications. Colourtemperatureremainsconstantasirradianceisincreasedwhichmakescolourbalancingunnecessaryandimprovesstandardisationbetweensessions.Sampleswillappearidenticaleventhoughirradiancecouldbeuptofivetimesgreater. Inaddition,thesemicroscopeilluminationsystemsdonotproduceunwantedlightintheUVwhichmakesitsaferandmorecomfortableespeciallyforlonghoursspentatthemicroscope,asriskofeyedamageissignificantlylower. Unlikehalogen,withLEDmicroscopeilluminationsystemstheavailabilityofnarrowbandwidthoptionsoptimisesimagequalitythroughincreasedcontrast.Theseevenrangeupto770nm,whichenablesvisualisingthroughthicktissuesamples. Findoutmore TherearemanybenefitstoadoptingthelatestLEDmicroscopeilluminationsystems.Formoreinformationonchoosingtherightmicroscopeilluminationsystemforyourneeds,contactusorfindyourlocalCoolLEDreseller. CustomMicroscopyIllumination Needsomethingalittledifferent? Formanufacturerswhowishtogainacompetitiveadvantagethroughbright,stableandcontrollableillumination,CoolLED’sworld-renownedLEDtechnologyisavailableincustomisedconfigurations. Streamlineddevelopmentusingprovenoff-the-shelftechnology Professionalsupport Market-leadingwarranty ‘Trybeforeyoubuy’option TrustedexpertsinLEDilluminationtechnologysince2006 LEARNMORE Environment&Safety DidyouknowthatLEDilluminationsystemshelplaboratoriesreducetheircarbonfootprint,protectinglaboratorystaffalongtheway? Toxicmercury-basedmicroscopeilluminatorsarebadnewsfortheenvironment,drawalotofpowerandhaveashortlifetime. LEDilluminationsystemsarecleaner,havealonglifetimeanduselesspower.OurpE-300SeriesisalsoACTlabelcertifiedandisanaturalchoiceforlabswhowanttoplaytheirpartinhelpingtheenvironment. LEARNMORE Help&Advice AtCoolLEDwearecommittedtogivingourcustomersoutstandingservicerightfromthestartandthroughoutthelifetimeoftheproduct.Pleasegetintouchifyouhaveanyquestionsorwouldlikeaquote.Youcanalsobrowseoursitefortechnicalresourcesandgeneralsupport. UK+44(0)1264323040 TickHeretoRequestaQuote Pleaseleavethisfieldempty. GotoTop Bycontinuingtobrowsethesiteyouareagreeingtoouruseofcookies.IUnderstand